FastQCFastQC Report
Mon 27 Feb 2023
SRR3128255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283309
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300221.3148461290171414No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA50210.21990015368046986No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC48260.2113599166823238No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG43290.18959326135884366No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG38440.16835215908140333No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA36700.1607316399138268No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35940.15740313728890834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95400.084.890751
CCGTACA21900.078.327663
CGTACAC22200.076.634094
ATCCGTA22850.076.197971
TCCGTAC23300.074.629782
CGTAGGG6750.074.498312
TAGGGCA9950.073.6831054
GAATAGG13900.072.109531
GTAGGGA16700.072.042693
ATAGGGC13550.071.101863
AGTAGGG35800.069.444692
AGATAGG13450.069.2740861
TAGGGTA6600.068.358694
GTACGGG4400.068.358682
AGGGATG30500.068.260635
GGTAAGG13200.068.090621
TAGCGGG13400.067.33842
TAAGGGA17700.067.175933
ATAGGGA20750.067.0409243
TAGGGCG2400.066.578514
Produced by FastQC (version 0.11.5)