FastQCFastQC Report
Mon 27 Feb 2023
SRR3128254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541550
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364391.4337313843914148No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC54090.21282288367334895No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG47750.18787747634317642No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG41130.1618303790993685No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40750.1603352285022919No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA29880.1175660522122327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113000.085.388171
CGTAGGG6350.074.0164952
GGTAAGG16100.069.8024751
GAATAGG16100.069.8024751
TAGGGCG2900.069.690354
CCGTACA14850.069.313833
TAGGGCA13000.068.692994
ATAGGGC14600.068.569183
GTAGGGC10150.068.532713
AAGAGGG88600.068.3257452
TAAGGGA24100.067.672883
AGTAAGG16500.067.540341
AAGGGAT39750.067.5151444
AGGGATG36000.067.49795
TAAGCGG6550.067.481591
TATAGGG17250.067.026762
TAGAGGG45950.066.485982
ATAGGGA24150.066.365063
AGACGGG14750.066.278632
AGTAGGG40550.065.8354342
Produced by FastQC (version 0.11.5)