FastQCFastQC Report
Mon 27 Feb 2023
SRR3128253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228490
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319821.4351421814771437No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC43760.19636614927596713No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG40080.17985272538804303No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35840.16082638916934786No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG34990.1570121472387132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101750.086.179721
TAGGGCA10900.078.116834
CGTAGGG5800.077.052742
GTACGGG4300.074.393152
GGTAAGG13900.072.125941
GAATAGG13550.070.862671
TAAGGGA20200.070.5640953
ATAGGGA20950.070.058863
AGGGTAC11000.069.2189255
GTAGGGA15700.069.215733
GTAGGGT8500.069.180483
ATAGAGG15350.068.685691
AGGGCAT22950.068.196815
TATAGGG18600.067.276162
ATAAGGG34800.067.1845862
ATAGGGC14800.067.067673
TAGAGGG39450.067.016512
AAGGGCG9100.066.686944
AGGGAAT20450.066.6510855
GTAGGGC8400.066.643873
Produced by FastQC (version 0.11.5)