FastQCFastQC Report
Mon 27 Feb 2023
SRR3128251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719018
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438221.6116848067942176No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC45050.16568481709205307No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG42230.1553134256558802No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40400.14858305461751264No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG34610.12728860198792358No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27900.10261057484724265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134050.085.731171
TAGGGCA13850.077.7109764
TAGGGTA7550.074.079274
GAATAGG18850.071.843171
ATAGGGC14750.071.694723
ATAGGGA22850.071.579673
AGGGATG43450.070.310515
GTAGGGA20300.070.152523
CGTAGGG8500.069.67042
AGTAGGG46400.068.7778242
ATAGAGG19500.068.724981
AGTAAGG15900.068.019761
TAGAGGG49900.067.8154452
GAGGGAT34900.067.60444
TATAGGG22000.067.50892
AAGGGTA17650.067.370924
AAGAGGG102800.067.162272
ACGGGTA2800.067.142684
TAGACGG3950.066.6646041
GATAGGG36450.066.405852
Produced by FastQC (version 0.11.5)