FastQCFastQC Report
Mon 27 Feb 2023
SRR3128250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363370
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384351.6262794230272875No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36040.15249410798986193No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG34640.146570363506349No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC33900.14343924142220643No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG29030.12283307311170066No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23800.10070365621972016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118200.085.157061
TAGGGCA11900.076.324854
ATAGGGC12300.072.694923
AGGGATG37050.071.549075
AGTAAGG13550.070.532161
GAATAGG13700.070.4472051
GAGGGAT30850.069.8660054
CGTAGGG7350.069.7917252
AAGGGAT42450.069.5097054
AGTAGGG39900.068.881842
ATAGGGA19400.068.649963
ACGGGTA3800.068.113734
GATAGGG33000.067.88252
GTACGGG5400.067.1060642
AGGGTAC9650.066.727735
GTAGGGA16400.066.286293
AGGGCAT20650.066.234775
GGTAAGG15300.065.849551
TAGAGGG43850.065.789372
AGATAGG12750.065.726461
Produced by FastQC (version 0.11.5)