FastQCFastQC Report
Mon 27 Feb 2023
SRR3128246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902183
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340811.7916782980396735No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG31250.16428492947313691TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC27860.14646330032389104TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT21880.11502573621991154No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG21790.11455259562302891TruSeq Adapter, Index 23 (95% over 22bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19900.10461664308849358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109100.086.530341
ATAGCGG4400.081.2873841
AGGGATG28850.070.047215
AGTAGGG30700.069.806312
CGTACAC9150.069.339534
GGTAAGG11800.067.401091
ATAGGGC10950.067.383543
CCGTACA9600.067.068343
CGTAGGG5500.066.6499942
ATCCGTA10000.066.356181
TAGGGCA10650.066.1926654
TAGCGGG11100.066.0495452
AAGAGGG70950.066.0405962
AAGGGCA30500.065.94964
GAGGGAT24500.065.795524
AGATAGG11550.065.600351
TCCGTAC10050.065.468172
AGAGGGC29150.065.456943
TAGGGTA5900.065.317584
ATAAGGG23550.065.256692
Produced by FastQC (version 0.11.5)