FastQCFastQC Report
Mon 27 Feb 2023
SRR3128245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124022
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408321.9223906343719603No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG36000.169489769879973TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC29940.14095899195017755TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT23640.11129828222118227No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG23350.10993294796381582TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT122500.086.0644451
ATAGCGG4350.074.590591
CGTAGGG5950.072.672512
AGTAGGG36550.070.9824452
AGGGATG31250.070.838635
ATAGGGC12850.070.2259143
ATAACGG1350.069.665941
TAGGGCA12150.068.082534
TAAGGGA17650.067.90393
TAGGGTA5900.067.712084
AGGGCAT21450.067.706515
GTAGGGC9650.067.2126543
TAGAGGG40250.067.1430742
AGATAGG13400.067.027471
CGAAGGG22800.066.583552
TCCGTAC6500.066.52332
GAATAGG12700.066.278631
AGGGTAT12350.066.2188345
AGGGAAT22200.066.054275
AGTAAGG13350.065.869541
Produced by FastQC (version 0.11.5)