FastQCFastQC Report
Mon 27 Feb 2023
SRR3128244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866882
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356931.9119044481654437No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG29020.15544635386703606TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC24350.13043138237981833TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT20890.11189780607451355No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG20110.10771971661840438TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104350.086.609661
ATAGCGG4850.073.7614441
AGTAAGG12550.072.0136951
ATAGGGC10050.070.6977163
AGAGGGC28350.070.539173
AGGGATG28750.069.314175
AGGGTAC8350.069.232925
AGTAGGG30650.068.930332
TAGGGTA6250.068.510314
TAGGGCA9700.068.397764
CGTAGGG5200.067.866032
AGATAGG9950.067.1773451
TAGAGGG34000.067.120832
ACTAGGG9150.066.852362
GGTAAGG12900.066.775781
AGGGAAT18700.066.603695
AAGAGGG68200.066.372052
TAGCGGG11650.066.23882
ATAGGGT8350.065.931643
GCATAGG6800.065.761581
Produced by FastQC (version 0.11.5)