FastQCFastQC Report
Mon 27 Feb 2023
SRR3128243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023832
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350471.7317148854252724No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC40450.19986836852070727No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG38970.19255550855999903No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29990.14818423663624253No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG27740.13706671304732804No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT25600.1264927128338716No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25180.12441744176394087No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACGGTGT23320.1152269555971049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115450.087.1916961
AGGGTAC16250.078.084965
TAGGGTA8950.076.663184
GAGGGAT24150.074.14214
AGATAGG11250.072.368881
AAGGGTA19650.071.748984
ATAGGGC12000.071.668253
GAATAGG12200.070.9767761
ATAGGGA18100.070.8828353
ACGGGTA3250.070.8548664
TAGGGCA9400.070.493374
AAGGGAT34200.070.493374
AGGGATC20600.069.808965
AGTAGGG35950.069.807062
AGTAAGG12850.069.58391
TAAGGGA19200.069.514293
GGTAAGG11250.069.440661
TCCGTAC15650.069.367272
CGTAGGG5900.069.298562
GTAGGGA15000.069.240153