FastQCFastQC Report
Mon 27 Feb 2023
SRR3128242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262180
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424231.8753149616741374No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC42690.18871177359891786No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG41230.1822578221008054No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG31810.14061657339380595No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29200.1290790299622488No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT26040.11511020343208764No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22700.10034568425147425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135450.087.125661
AGGGTAC18550.078.5461965
TAGGGTA9350.076.408094
GAGGGAT28900.074.3234254
TAAACGG1900.074.236761
GAATAGG12600.072.390661
AGATAGG11900.071.5126651
AAGGGTA22650.071.383464
AGTAAGG13250.071.3233261
GTACGGG4350.071.31192
AGGGATG28000.071.005125
AGTAGGG38900.070.561952
GGTAAGG13650.070.266591
ATAGGGC12850.069.861453
ATAGGGA21150.069.779313
AGGGCAT17350.069.621125
GATAGGG31000.069.591852
GAGGGTA16850.069.455534
GGGTACC19550.069.2393656
ACGGGTA3400.069.1191564
Produced by FastQC (version 0.11.5)