FastQCFastQC Report
Mon 27 Feb 2023
SRR3128241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949615
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366351.8790889483308242No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC36620.1878319565657835No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG34930.17916357845010425No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG26430.13556522698071158No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT24130.12376802599487591No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23680.12145987797590806No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20670.10602093233792313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116000.087.637891
TAGGGTA7300.078.0215764
ACGGGTA2850.077.625654
AGGGTAC16000.077.549415
ATAGCGG4050.076.733711
ATAGGGA17350.076.507123
AGGGATG24600.074.320575
AAGGGTA19850.072.799794
TAGGGCA8500.072.544524
GAGGGAT25200.071.9138264
CGTAGGG5600.071.448692
AAGGGAT35000.071.009774
TAGAGGG37950.070.701142
AGTAGGG32700.070.536152
AGATAGG10550.070.518321
GAGGGTA15250.070.0661544
GGTAAGG11400.069.803811
GTAGGGA14950.069.583033
GAATAGG10050.069.341451
TAGCGGG9850.069.293922
Produced by FastQC (version 0.11.5)