FastQCFastQC Report
Mon 27 Feb 2023
SRR3128239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922127
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481811.6488331958193467No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC60760.2079307299100963No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG53780.18404401998954872TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG46450.15895955240822865TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38180.13065824996654835No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34900.11943354960273801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151350.086.225721
TAGGGTA8250.075.771994
TAGGGCA16550.073.839094
AGGGTAC12550.072.2810755
AGGGATG45200.071.9579245
CGTAGGG7750.071.564682
ACGGGTA2850.070.914424
GTAGGGA19400.070.5033343
AGGGCAT27550.070.118275
AAGGGAT49550.068.960714
GTAGGGT10500.068.93663
ATAGGGA29050.068.7640153
GTACGGG7250.068.720492
GAGGGAT36800.068.586314
GGTAAGG16950.068.530441
ATAGGGC18300.068.3201753
AAGAGGG104100.067.907162
AAGGGTA19750.067.824844
GAGGGTA15500.067.318164
ACGGGAT10550.067.272184
Produced by FastQC (version 0.11.5)