FastQCFastQC Report
Mon 27 Feb 2023
SRR3128238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546773
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415851.6328506702403396No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC49190.19314638564175135No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG44450.17453459731197085TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG37670.14791267223266463TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34080.1338164021685482No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29550.1160291867394542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134900.086.512881
TACGGGT1650.076.988693
ACGGGTA2650.076.343084
AGGGCAT25650.074.950335
TAGGGCA16200.074.929324
AGGGTAC10900.072.878235
ATAGGGC15850.072.725043
AAGGGTA16900.072.382534
AGGGATG39000.071.9527365
GAATAGG13450.070.67571
GTAGGGC11650.070.673933
TAGGGTA8500.069.742694
CGAGGGA15300.069.1891863
AGTAGGG37200.069.181752
AAGGGAT46900.068.817414
AGGGTAG9300.068.737475
TAGCGGG11700.068.361282
AGGGAAT26200.068.3535845
GTAGGGA15300.068.266673
AGGGACT33850.067.902825
Produced by FastQC (version 0.11.5)