FastQCFastQC Report
Mon 27 Feb 2023
SRR3128237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187525
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283811.2974023154021097No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTTTGTC72220.33014479834516175No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG61060.27912823853441676RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC61020.27894538348133163TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG54110.2473571730608793RNA PCR Primer, Index 35 (95% over 23bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA46750.21371184329321952No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38520.1760894161209586No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28410.12987280145369767No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATG22200.10148455446223471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98000.086.2889561
ATAGCGG4750.077.274151
CCGTACA30700.075.164053
TAGGGCA11500.074.785994
TCCGTAC32200.074.14382
AGGGATG30200.072.6736455
ATCCGTA33350.072.103821
AGGGCAT23100.071.4105155
CGTACAC32300.071.004244
CGTAGGG6050.070.689192
GGTAAGG11900.070.389241
ACGGGTA2550.070.034254
AGGGAAT20750.069.532425
AGTAGGG32200.069.473332
AGAGGGC31150.068.495933
ATAGGGA17750.068.310643
TAGAGGG36000.068.014612
AGTTAGG6450.067.8510361
ACGGGAT7500.067.675194
ATAGGGC13150.067.546543
Produced by FastQC (version 0.11.5)