FastQCFastQC Report
Mon 27 Feb 2023
SRR3128235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115704
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296781.4027482105247238No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG55430.26199317106740827RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC54430.25726661196462264TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG46910.2217228875116746RNA PCR Primer, Index 35 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36750.17370104702737243No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28880.13650302688844942No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTTTGTC23850.11272843460143764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98600.086.705031
ATAGCGG5250.086.082981
TAGGGCA11050.078.784374
CGTAGGG6400.076.468752
AGGGCAT21200.073.161895
AGTAGGG31300.071.714122
GGTAAGG11800.071.412861
TAGCGGG12200.070.586532
GTAGGGC6750.069.7150963
ATAGGGC13300.069.7019963
AAGGGCA36100.069.217834
AGGGATG31050.069.025575
AGGGCAC16800.068.8228455
GTAGGGA16900.068.776633
ATAGGGA16400.068.577983
CGAGGGA12850.068.480843
TAGAGGG32400.068.26272
AAGGGAT38950.067.898394
GTACGGG4300.067.8506242
AAGGGTA14650.067.7759254
Produced by FastQC (version 0.11.5)