FastQCFastQC Report
Mon 27 Feb 2023
SRR3128234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139360
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309901.4485640565402738No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG42480.1985640565402737RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC39450.18440094233789545RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG37500.17528606686111733Illumina PCR Primer Index 7 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGTCCACC34790.16261872709595393No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA30990.14485640565402738No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24840.11610949068880413No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21710.1014789469747962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93050.086.3292541
ACGGGTA2100.076.087164
CGTAGGG5700.074.2026442
TAGGGCA10800.072.233074
CCGTACA17800.072.0766143
TCCGTAC18500.071.889662
CGTACAC18100.070.881984
AGTAGGG35200.069.691472
GTAGGGA16750.068.738973
GAATAGG13350.068.7375641
GGTAAGG11250.068.601271
AGGGATG31350.068.506295
AGTAAGG13400.068.4810941
TAGAGGG39250.068.247532
ATAGCGG4000.068.235261
ATCCGTA19800.067.973821
AAGGGTA14600.067.273684
AGAGGGC30900.067.070553
AGGGTAC9300.066.70265
TAAGGGA19050.066.60713
Produced by FastQC (version 0.11.5)