FastQCFastQC Report
Mon 27 Feb 2023
SRR3128233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382644
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368231.5454679759124739No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG45460.19079644294321768RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC42240.177282044652915RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG39820.16712526084467508Illumina PCR Primer Index 7 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28440.11936319483733196No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24810.10412801912497208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114150.086.183391
CGTAGGG6300.079.0798952
ATAGCGG5500.074.3865361
AGGGATG37650.072.2778555
TAGGGCA13500.071.3693544
GTAGGGA17950.070.696853
TAGAGGG44750.070.474222
AGTAGGG39450.070.1728442
TAGGGTA9450.070.125984
GAGGGAT32250.069.369554
ATAGGGA20550.068.156173
AGGGTAC9700.067.834085
AGGGTAT13850.067.5297245
GTACGGG5650.067.380982
AAGGGAT39850.067.344084
CCGTACA12700.066.98473
AGTAAGG14550.066.902931
AAGGGTA16650.066.899944
TAGCGGG14250.066.295182
AGGGAAT23350.066.221755
Produced by FastQC (version 0.11.5)