FastQCFastQC Report
Mon 27 Feb 2023
SRR3128232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067515
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325151.5726608996790834No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG38160.18456939852915213RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC35870.17349329992769097RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG33330.1612080202562013Illumina PCR Primer Index 7 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25070.12125667770245922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101500.086.526311
CGTAGGG6350.073.356382
TAGGGCG3600.071.882974
AGTAGGG35800.071.629162
TATAGGG15750.071.399262
TAGGGTA8250.070.148284
GTACGGG3900.069.974492
TAGGGCA11400.069.7505654
ATAGGGC13450.069.614013
AGGGATG31550.069.566475
GTAGGGA16200.068.83343
ATAACGG1850.068.690171
TAGCGGG10900.068.6352162
TAGAGGG34900.068.488032
AAGGGTA15450.067.606794
GTAGGGC10150.066.75173
ATAGGGA19050.066.6863
TAAGGGA19900.066.201953
AGGGTAT10650.065.753535
AAGGGAT36250.065.4166644
Produced by FastQC (version 0.11.5)