FastQCFastQC Report
Mon 27 Feb 2023
SRR3128230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962261
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450171.519683782084023No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58690.1981256884521654No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45570.15383519548074934No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC41940.14158104231868834No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG39460.13320905889116455No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG35760.12071859974526215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139350.085.668531
AGGGATG44050.073.406115
TAGGGTA8750.071.9757844
AGTAGGG50800.070.313632
TAAGGGA22600.070.082753
AAGGGAT48350.069.8911364
GGTAAGG18050.069.81091
ATAGAGG20500.069.265781
AGAGGGC39000.069.052543
AGGGTAA13550.069.024535
TAGAGGG56200.068.157082
TATAGGG25150.068.0225752
AAGGGTA19750.067.583544
GTAGGGT12650.067.247743
GAGGGAT36150.067.085884
ATAGGGA26000.066.341063
ATGAGGG47250.066.1469042
AAGAGGG113300.066.080832
ATAGGGC16800.065.18333
AGGGACT43800.065.133485
Produced by FastQC (version 0.11.5)