FastQCFastQC Report
Mon 27 Feb 2023
SRR3128229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482613
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389991.5708851923356562No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45380.18279127677169177No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC38450.15487713953000326No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37530.15117136662057276No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG33730.13586491329901199No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG29450.11862501324209614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118850.085.9072951
AGGGATG37900.073.410925
CGTAGGG8650.072.901382
GAATAGG14500.071.7453841
ATAGGGA21300.070.477323
AGTAGGG45700.070.22882
GAGGGAT30000.068.7054444
AAGGGTA16550.068.241974
TAGAGGG44650.068.0860442
AGAGGGC35050.068.070393
AGTAAGG14300.067.811111
AGGGACT35350.067.671615
GTAGGGT11700.067.571213
TAGGGTC6650.067.226464
TAGGGCA12750.067.173744
GGTAAGG14450.067.107191
AAGGGAT41800.066.53494
TATAGGG17850.066.43692
TAGGGTA8650.066.371564
ATAGGGC13300.066.164993
Produced by FastQC (version 0.11.5)