FastQCFastQC Report
Mon 27 Feb 2023
SRR3128228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195928
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357211.6266926784484739No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC30860.14053284078530806No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG30360.13825589910051694No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29670.1351137195755052No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29260.13324662739397647No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24110.10979412804062792No Hit
CGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT23900.10883781253301565No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGGACTGG23470.10687964268409529No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG22000.1001854341308094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110800.085.0277561
GGTAAGG15500.074.078911
ATAGGGC13150.073.980453
TAGGGCA10900.073.2984544
GAGGGAT27150.072.183624
TAGACGG2950.071.7838751
TAGGGTA6150.071.069014
AGGGATG33850.070.25295
CCGTACA12850.069.855833
ATAGGGA18750.069.6812443
AGGGAAT20000.068.3815
TCCGTAC13550.068.32812
AGTAGGG36200.068.288822
AGTAAGG12700.067.808451
AGGGCAT21350.067.579185
CGTACAC13300.067.1389164
AGATAGG13200.067.022461
ATAGAGG16050.066.8492051
GTAGGGT8550.066.510723
AGGGATC17100.066.510725