FastQCFastQC Report
Mon 27 Feb 2023
SRR3128227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446102
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426971.7455118388358295No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34340.14038662328880808No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34250.14001869096219208No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG33850.13838343617723217No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC31980.13073862005754464No Hit
CGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT25460.10408396706269812No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG24860.10163108488525825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134700.084.721051
TAGACGG3900.073.5452041
AGGGATG35900.072.5256045
TAGGGCA14200.070.496454
GAGGGAT29900.070.103514
ATAGGGC16300.069.488723
GGTAAGG16650.069.472031
TAAGGGA22350.069.3939063
AGATAGG15600.069.32541
ATAGCGG6350.068.864971
ATAGAGG17000.068.594911
TAGAGGG43550.068.420462
AGTAGGG41150.068.29932
AGGGATA21500.068.2012255
TAGGGAT15950.067.181584
ATAGGGA22950.067.170113
TAGGGTA7600.066.7861254
GAATAGG13900.066.640861
CGTAGGG6400.066.091782
AGTAAGG14450.066.056761
Produced by FastQC (version 0.11.5)