FastQCFastQC Report
Mon 27 Feb 2023
SRR3128226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133736
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381301.7870064525320848No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29900.14012980050015558No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG29250.13708350048928264No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC29170.13670857125717523No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27710.12986611277121443No Hit
CGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT24290.11383788809862139No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG22350.10474585422001598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119800.084.524921
ATAGGGC13250.075.300663
AGGGATG31400.074.84615
GAGGGAT24550.070.3547444
TAGACGG3750.070.3004151
TAAGGGA18150.069.751643
TAGGGCA11600.069.377234
AGGGAAT21150.068.89385
ATAGGGA19650.068.7382053
AGATAGG12700.068.575521
GTAGGGA15900.068.374423
AGGGCAT19550.067.800235
AAGGGAT35750.067.796924
AGGGATA20200.067.480065
GGTAAGG16200.067.4177251
TAGCGGG10400.066.974152
ATAGAGG16300.066.715311
AGAAGGG80550.066.6067352
AGGGTAC9750.066.5278245
AGTAAGG12750.066.460471
Produced by FastQC (version 0.11.5)