SRR3128225.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128225.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1912817
Sequences flagged as poor quality	0
Sequence length	100
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22088	1.154736705079472	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5737	0.2999241432923275	TruSeq Adapter, Index 10 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGATTACC	5591	0.2922914215003317	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	5146	0.2690273037096596	RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	4669	0.2440902605947145	Illumina PCR Primer Index 2 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA	4602	0.24058757319701782	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	4283	0.22391059887067083	No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	2090	0.1092629352415835	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7910	84.209366	1
ATAGCGG	435	80.02323	1
TAGGGTA	695	78.44006	4
AGGGATG	3605	77.697266	5
CGTAGGG	450	76.23863	2
TAGGGCA	1150	76.0116	4
TCCGTAC	2530	75.97445	2
CCGTACA	2490	75.118774	3
AAGGGAT	3835	74.630554	4
CGTACAC	2515	74.37206	4
ATAGGGC	1080	73.975845	3
AAGGGAC	2210	73.152794	4
ATCCGTA	2665	72.723305	1
GAATAGG	1160	72.18312	1
AGGGTAC	960	71.963264	5
ATAGGGA	1935	71.40541	3
AAGGGCG	725	71.30491	4
AGGGATT	2275	70.85614	5
GAGGGAT	3085	70.532715	4
GTAGGGA	1620	70.49463	3