SRR3128224.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3128224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2123623
Sequences flagged as poor quality	0
Sequence length	100
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26846	1.2641603523789298	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	6321	0.297651701832199	TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	5857	0.2758022492692912	RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	5089	0.23963763813068514	Illumina PCR Primer Index 2 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	4902	0.23083193203313393	No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA	3296	0.15520645613651765	No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGATTACC	3203	0.15082714775645206	No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	2222	0.10463250774737323	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9015	85.96982	1
AGGGATG	4310	77.753746	5
CGTAGGG	490	76.73668	2
TAGGGCG	285	75.8616	4
TAGGGTA	775	74.59547	4
TAGGGCA	1325	73.42831	4
GAATAGG	1340	73.34931	1
AAGGGAT	4425	72.971375	4
ATAGGGA	2225	72.667046	3
AGGGTAA	1090	71.579834	5
GAGGGAT	3530	70.967834	4
ACGGGTC	265	70.94523	4
AGGGTAC	895	70.89568	5
AGTAGGG	3790	70.3158	2
AGGGCAT	2055	70.21592	5
ATAGGGC	1205	69.81923	3
GTAGGGA	2025	69.39932	3
TAGCGGG	1085	69.31054	2
TAGAGGG	3425	69.301056	2
AGAGGGC	2780	69.14927	3