FastQCFastQC Report
Mon 27 Feb 2023
SRR3128223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875237
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232521.2399499369946305No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC51370.2739387074807078TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG47790.25484778723969287RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG42390.2260514270996146Illumina PCR Primer Index 2 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41970.22381171019983073No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT19890.10606659318262172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75550.084.8004761
GTACGGG3850.083.120252
AGGGATG35550.079.3299565
ATAGCGG3650.078.670441
TACGGGT1300.076.021183
TAGGGCA11500.075.706384
TAGGGTA7750.075.2971654
GTAGGGA16400.074.8954243
AAGGGAT37550.074.6955954
TAGCGGG9550.073.424592
CGAAGGG16100.073.075662
AGGGTAC9000.072.593525
ATAGGGA21000.072.383883
CGTAGGG5000.071.532312
AAGGGTA13350.070.8554844
GAATAGG11050.069.864441
ACGGGTA2500.069.649884
GAGGGAT31600.069.101834
AGAGGGA46250.069.090243
GGATGGC27650.068.837897
Produced by FastQC (version 0.11.5)