FastQCFastQC Report
Mon 27 Feb 2023
SRR3128222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078517
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289011.3904625268881612No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG32090.15438892248656133No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC31400.15106924793013482No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGGTGGCT31230.15025135709739204No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31200.15010702342102566No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30270.14563267945366817No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG24260.11671783295493854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93650.084.825061
GTAGGGA15000.072.6893543
TCCGTAC16600.071.345492
ATAGGGC12950.071.131233
CGTAGGG6750.071.018332
AGGGATG30350.070.612285
ATAGAGG15550.070.515581
ATCCGTA17100.069.903211
AGTAGGG34150.069.498392
CCGTACA16800.069.3771443
CGAAGGG21700.069.088382
TAGGGCA9700.068.800364
GAATAGG13550.068.767841
ATAGGGA16200.068.755493
CGTACAC17050.068.084254
TAAGGGA19050.067.8440553
AAGGGAT32750.067.7336964
ATAAGGG30250.066.806262
TATAGGG16250.066.519432
AGTAAGG12950.066.1395341
Produced by FastQC (version 0.11.5)