FastQCFastQC Report
Mon 27 Feb 2023
SRR3128221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300150
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343941.4952937851879224No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG35190.15299002238984416No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC33770.14681651196661086No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33490.14559920005217053No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG26230.11403604112775255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112850.085.74241
TAGGGTA7350.076.0934
CGTAGGG7050.075.999432
ATAGCGG5000.072.4408651
GGTAAGG11700.071.966451
TAGACGG2900.071.3703161
AGGGATG31100.071.026855
AGTAAGG14700.070.7192841
TAGGGCA11200.070.4979254
GTAGGGT9750.069.8969043
AGTAGGG42200.068.940232
TAGGGTC6350.068.832624
GAATAGG14550.068.538681
AGGGCAT18300.068.0581055
ATAGAGG18800.067.556761
ATAGGGA20700.067.434273
GTAGGGA16900.067.301263
AAGAGGG83200.067.110062
ATAAGGG32250.066.892522
AGAGGGC29600.066.6886753
Produced by FastQC (version 0.11.5)