FastQCFastQC Report
Mon 27 Feb 2023
SRR3128220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989120
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311331.565164494851995No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG31750.15961832368082368No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30100.1513231981981982No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC28540.1434805341055341No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG23040.11583011583011582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97550.086.32561
CGTAGGG6550.076.145842
TAGGGCA11050.073.239914
ATAGGGC14700.072.339043
GGTAAGG10900.072.118471
AGATAGG10800.071.47871
AGGGATG26950.068.535085
AGTAAGG12700.068.1978451
GTAGGGA14700.068.177953
GTACGGG4850.067.910662
AGAGGGC26050.067.553143
ATAGCGG4400.067.39761
TAGAGGG34750.067.295072
TAAGAGG13650.067.244771
AAGAGGG69750.067.188782
GAATAGG12150.067.023391
AGAGGGA44750.066.977363
TAAGGGA18500.065.873343
AGTAGGG33400.065.788812
AAGGGAT31050.065.312654
Produced by FastQC (version 0.11.5)