FastQCFastQC Report
Mon 27 Feb 2023
SRR3128218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383822
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322311.3520724282266041No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC43540.18264786548660095TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG43440.1822283710780419No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41590.17446772451969988No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG39040.16377061710144467No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25840.10839735517165293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101900.085.599071
TAGGGCA13350.076.4003754
GTAGGGC10350.073.114353
AGTAGGG39450.072.320052
GAATAGG13600.071.22421
CGTAGGG6950.071.010332
GGTAAGG14400.070.8592451
TAAGGGA19650.069.845323
ATAGGGC13300.068.559423
AAGAGGG85000.068.125372
GAGGGAT27450.067.8062744
ATAGGGA21250.067.682993
AGGGATG32450.067.6410145
ATAGAGG16000.067.593841
GTAGGGA17600.067.565483
TAGCGGG13200.066.586272
AGAGGGC32650.066.076423
ATAAGGG31700.065.9808352
AGGGTAC11350.065.842935
TAGAGGG44050.065.8349152
Produced by FastQC (version 0.11.5)