FastQCFastQC Report
Mon 27 Feb 2023
SRR3128217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059876
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277111.345275152484907No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36800.17865153048047552No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC36110.17530181428396663TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG34990.16986459379108257No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG32140.15602880950115444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86900.086.35811
GAATAGG11700.076.856761
ATAGGGA17950.075.5136263
TAGGGCA11950.072.862014
ATAGGGC12600.072.09153
AATAGGG29600.070.756382
GGTAAGG10700.069.9596941
AGTAGGG32350.068.9606552
AGGGATG28750.068.3319555
GTAGGGA15550.067.79773
GATAGGG29600.067.0993042
CGTAGGG5350.066.8585742
AGGGCAT18500.066.560265
TAGAGGG37800.066.488532
AGACGGG11850.066.327772
GTAGGGC8450.066.280783
ATAGAGG13100.066.127341
AGATAGG11250.066.120961
TAAGGGA17250.066.02733
TAGGGAT13850.065.585054
Produced by FastQC (version 0.11.5)