FastQCFastQC Report
Mon 27 Feb 2023
SRR3128214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135645
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299641.4030421722711406No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG49400.23131185192295536No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC47130.2206827445572649No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG38960.18242732289308383No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30960.14496791367479145No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26930.12609773628107668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98150.086.584711
CGTAGGG7600.079.231552
TAGGGCA11600.078.676594
AGGGATG33950.073.0935065
AGTAGGG34150.072.873012
AGGGCAT21050.072.563125
ATAGCGG3650.072.19531
GAGGGAT30450.071.685694
CGAAGGG23800.071.1585852
GAATAGG12650.071.0487751
CGAGGGA10800.070.56563
GTACGGG5600.070.56562
TAGAGGG37250.070.218292
ATAGGGA18500.070.184163
GTAGGGT8650.069.6138463
GGTAAGG12650.069.560841
AGTACGG3050.069.426681
AGAGGGC28000.069.22153
AGGGAAT22500.068.931245
GTAGGGA17100.068.777383
Produced by FastQC (version 0.11.5)