Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128213.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1064117 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 13123 | 1.2332290528203196 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGGCAAC | 4561 | 0.4286182816363239 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 3633 | 0.34140982617512927 | RNA PCR Primer, Index 34 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 2946 | 0.27684925623780093 | RNA PCR Primer, Index 34 (96% over 25bp) |
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 2682 | 0.25203995425315073 | TruSeq Adapter, Index 18 (95% over 22bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2668 | 0.25072430945093443 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 2447 | 0.2299559165016629 | No Hit |
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATG | 1383 | 0.12996691153322426 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT | 1178 | 0.11070211264362848 | RNA PCR Primer, Index 34 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 5580 | 0.0 | 88.27555 | 1 |
CTAACGG | 85 | 0.0 | 83.02335 | 1 |
ATAGCGG | 180 | 0.0 | 81.02464 | 1 |
CGAAGGG | 990 | 0.0 | 75.95269 | 2 |
CGTAGGG | 300 | 0.0 | 75.19315 | 2 |
TAGCGGG | 555 | 0.0 | 74.51574 | 2 |
CCGTACA | 1975 | 0.0 | 74.4793 | 3 |
TCCGTAC | 2095 | 0.0 | 73.80235 | 2 |
AGGGATG | 2065 | 0.0 | 73.50905 | 5 |
AGGGAAT | 1255 | 0.0 | 72.646774 | 5 |
CGTACAC | 2015 | 0.0 | 72.53434 | 4 |
TAACGGG | 350 | 0.0 | 72.50769 | 2 |
TAGGGCA | 590 | 0.0 | 72.48493 | 4 |
TAGGGCG | 150 | 0.0 | 72.060104 | 4 |
AGTAGGG | 1645 | 0.0 | 70.56501 | 2 |
CTACGAC | 480 | 0.0 | 70.49359 | 5 |
GTAAGGG | 1550 | 0.0 | 70.34199 | 2 |
TGTAGGG | 675 | 0.0 | 70.31953 | 2 |
AAGAGGG | 3755 | 0.0 | 70.08683 | 2 |
ATAAGGG | 1315 | 0.0 | 70.04687 | 2 |