FastQCFastQC Report
Mon 27 Feb 2023
SRR3128213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064117
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131231.2332290528203196No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGGCAAC45610.4286182816363239No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG36330.34140982617512927RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC29460.27684925623780093RNA PCR Primer, Index 34 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG26820.25203995425315073TruSeq Adapter, Index 18 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26680.25072430945093443No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24470.2299559165016629No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATG13830.12996691153322426No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT11780.11070211264362848RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55800.088.275551
CTAACGG850.083.023351
ATAGCGG1800.081.024641
CGAAGGG9900.075.952692
CGTAGGG3000.075.193152
TAGCGGG5550.074.515742
CCGTACA19750.074.47933
TCCGTAC20950.073.802352
AGGGATG20650.073.509055
AGGGAAT12550.072.6467745
CGTACAC20150.072.534344
TAACGGG3500.072.507692
TAGGGCA5900.072.484934
TAGGGCG1500.072.0601044
AGTAGGG16450.070.565012
CTACGAC4800.070.493595
GTAAGGG15500.070.341992
TGTAGGG6750.070.319532
AAGAGGG37550.070.086832
ATAAGGG13150.070.046872