FastQCFastQC Report
Mon 27 Feb 2023
SRR3128212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188189
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159001.3381709475512733No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG37830.31838369148342566RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC30520.2568614925739929RNA PCR Primer, Index 34 (96% over 25bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29820.2509701739369747No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG28290.23809343463034924TruSeq Adapter, Index 18 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGGCAAC26410.22227103600521467No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17760.1494711699906328No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT13250.11151424562927277RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1850.088.917214
CGTTTTT63950.087.858011
CGAAGGG11250.080.6295552
AGGGATG26000.077.367745
ATAGCGG2350.076.027331
AGGGCAT12350.073.828545
TAGGGCA7400.073.674264
AAGGGCA18500.073.166154
AAGGGAT26550.072.5786364
TCCGCTA5850.072.3336941
GCGGGAT7150.072.30634
TACGGGT1050.071.617673
CGTAGGG3550.071.491582
AGTAGGG18250.071.335612
GAAGGGT11550.071.210753
TAGCGGG6850.069.9840552
GAGGGAT19300.069.889854
AAGAGGG37300.069.553632
AGAGGGC16050.069.4005363
CTACGAC6050.069.139165