Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128212.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1188189 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 15900 | 1.3381709475512733 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 3783 | 0.31838369148342566 | RNA PCR Primer, Index 34 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 3052 | 0.2568614925739929 | RNA PCR Primer, Index 34 (96% over 25bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2982 | 0.2509701739369747 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 2829 | 0.23809343463034924 | TruSeq Adapter, Index 18 (95% over 22bp) |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGGCAAC | 2641 | 0.22227103600521467 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 1776 | 0.1494711699906328 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT | 1325 | 0.11151424562927277 | RNA PCR Primer, Index 34 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAGGGCG | 185 | 0.0 | 88.91721 | 4 |
CGTTTTT | 6395 | 0.0 | 87.85801 | 1 |
CGAAGGG | 1125 | 0.0 | 80.629555 | 2 |
AGGGATG | 2600 | 0.0 | 77.36774 | 5 |
ATAGCGG | 235 | 0.0 | 76.02733 | 1 |
AGGGCAT | 1235 | 0.0 | 73.82854 | 5 |
TAGGGCA | 740 | 0.0 | 73.67426 | 4 |
AAGGGCA | 1850 | 0.0 | 73.16615 | 4 |
AAGGGAT | 2655 | 0.0 | 72.578636 | 4 |
TCCGCTA | 585 | 0.0 | 72.333694 | 1 |
GCGGGAT | 715 | 0.0 | 72.3063 | 4 |
TACGGGT | 105 | 0.0 | 71.61767 | 3 |
CGTAGGG | 355 | 0.0 | 71.49158 | 2 |
AGTAGGG | 1825 | 0.0 | 71.33561 | 2 |
GAAGGGT | 1155 | 0.0 | 71.21075 | 3 |
TAGCGGG | 685 | 0.0 | 69.984055 | 2 |
GAGGGAT | 1930 | 0.0 | 69.88985 | 4 |
AAGAGGG | 3730 | 0.0 | 69.55363 | 2 |
AGAGGGC | 1605 | 0.0 | 69.400536 | 3 |
CTACGAC | 605 | 0.0 | 69.13916 | 5 |