FastQCFastQC Report
Mon 27 Feb 2023
SRR3128211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011883
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140321.3867215873771968No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG33800.33403071303698156RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC27350.27028816572666997RNA PCR Primer, Index 34 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG24410.24123342323173727TruSeq Adapter, Index 18 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23910.2362921404945038No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGGCAAC14120.13954182449947278No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT11810.11671309825345419RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55500.087.76941
TAGGGCA6350.077.785114
CGAAGGG9500.076.751882
TAGGGCG2150.076.579154
AGGGTAT6700.076.462095
ACGGGTA1050.076.1623844
AGGGATG22650.076.153935
GTACGGG2400.074.482332
TACGGGT700.073.922323
CGAGGGA6550.073.2554243
TAGCGGG5350.072.980232
AAGGGAT21550.072.908824
AAGGGCA18900.072.428934
CGTAGGG2750.071.845152
TCCGCTA4800.071.5776441
AGGGATT12650.071.33545
GAGGGAT16900.071.258094
ATAGGGA10500.071.234233
AGGGCGT2250.071.021675
AAGGGTA7700.070.8676764