Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128211.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1011883 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 14032 | 1.3867215873771968 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 3380 | 0.33403071303698156 | RNA PCR Primer, Index 34 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 2735 | 0.27028816572666997 | RNA PCR Primer, Index 34 (96% over 25bp) |
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 2441 | 0.24123342323173727 | TruSeq Adapter, Index 18 (95% over 22bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2391 | 0.2362921404945038 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTGGCAAC | 1412 | 0.13954182449947278 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT | 1181 | 0.11671309825345419 | RNA PCR Primer, Index 34 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 5550 | 0.0 | 87.7694 | 1 |
TAGGGCA | 635 | 0.0 | 77.78511 | 4 |
CGAAGGG | 950 | 0.0 | 76.75188 | 2 |
TAGGGCG | 215 | 0.0 | 76.57915 | 4 |
AGGGTAT | 670 | 0.0 | 76.46209 | 5 |
ACGGGTA | 105 | 0.0 | 76.162384 | 4 |
AGGGATG | 2265 | 0.0 | 76.15393 | 5 |
GTACGGG | 240 | 0.0 | 74.48233 | 2 |
TACGGGT | 70 | 0.0 | 73.92232 | 3 |
CGAGGGA | 655 | 0.0 | 73.255424 | 3 |
TAGCGGG | 535 | 0.0 | 72.98023 | 2 |
AAGGGAT | 2155 | 0.0 | 72.90882 | 4 |
AAGGGCA | 1890 | 0.0 | 72.42893 | 4 |
CGTAGGG | 275 | 0.0 | 71.84515 | 2 |
TCCGCTA | 480 | 0.0 | 71.577644 | 1 |
AGGGATT | 1265 | 0.0 | 71.3354 | 5 |
GAGGGAT | 1690 | 0.0 | 71.25809 | 4 |
ATAGGGA | 1050 | 0.0 | 71.23423 | 3 |
AGGGCGT | 225 | 0.0 | 71.02167 | 5 |
AAGGGTA | 770 | 0.0 | 70.867676 | 4 |