FastQCFastQC Report
Mon 27 Feb 2023
SRR3128210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164944
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293981.3579104124633246No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGGGTGA42570.1966332616455668No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA39510.1824989468549764No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39100.1806051334353221No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG36420.16822606035075272No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC35240.16277557294784528No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG30310.14000362134078295No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23040.10642307607032792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94800.086.363951
ATAGGGC15250.078.2768863
AGGGATG33300.074.9410865
CCGTACA22400.074.90143
TCCGTAC23350.074.470542
GTAGGGA16100.074.4361653
TAGGGCA11700.073.909744
AGTAAGG13050.072.112581
CGTACAC23150.072.068764
AGGGAAT20800.071.851115
AGGGTAC8400.071.61445
GAATAGG12700.071.135951
TAAGGGA17450.070.832083
GAGGGAT28600.070.824084
AGTAGGG34200.070.632842
ATCCGTA24950.069.5900961
AAGGGAT37200.069.3584064
ACGGGTA1900.069.258674
TAGGGTA5300.069.165324
AGATAGG12200.069.037461