FastQCFastQC Report
Mon 27 Feb 2023
SRR3128209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390929
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347771.4545392188559343No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44700.18695661811789474No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG37280.1559226560052599No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC36120.15107098537848676No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG31490.13170612761817688No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27520.11510170314551374No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25800.10790784669891913No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGGGTGA25130.10510558866448982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111800.086.012141
AGGGATG40650.075.968125
ACGGGTA2450.074.822844
TAGGGCA13450.074.088234
TAAGGGA19800.073.829663
GGTAAGG15100.072.8697361
ATAGGGC14900.071.610263
AGTAGGG38400.071.485392
GAGGGAT31900.071.46394
CGTAGGG7700.071.42182
GAATAGG12400.070.534161
AGTAAGG12350.069.6774751
ATAGGGA20550.069.534033
AGATAGG13400.069.4814151
TAGAGGG45650.068.987372
TAGGGTA8050.068.90044
AGGGTAC10100.068.875845
AGGGAAT25000.068.8125465
AAGGGTA14550.068.810114
ATAGAGG19850.067.7506941
Produced by FastQC (version 0.11.5)