FastQCFastQC Report
Mon 27 Feb 2023
SRR3128208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075187
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312161.5042499784356784No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37130.17892363435198852No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG32740.1577689143195288No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC32130.1548294201920116No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG27440.13222904730995327No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23960.11545947425460935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102350.087.869271
ACGGGTA2050.080.329234
TAGGGCA11300.076.612364
AGGGATG34850.076.198155
ATAGCGG3700.073.771821
AGTAGGG35650.073.379492
GAATAGG11850.072.6769941
AAGGGTA13050.072.467794
ATAGGGC14950.072.069873
GAGGGAT29900.071.597794
AAGGGAT36700.071.408284
AGGGAAT21050.071.22585
TAGCGGG9850.070.694382
GTACGGG5350.070.355112
TAAGGGA19650.069.916513
CGTAGGG5650.069.1176762
AGAGGGA49750.068.6598743
CGAGGGA12550.068.231853
GGTAAGG11500.067.932011
AGATAGG12700.067.812791
Produced by FastQC (version 0.11.5)