FastQCFastQC Report
Mon 27 Feb 2023
SRR3128206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685494
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482271.7958334667662637No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG47120.17546120006226043No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC46920.1747164581265123No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG36820.13710699037123153No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32450.12083437907513478No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT30980.115360525847386No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29000.10798758068347947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT144950.087.2979351
TAGGGCA13300.074.565364
ATAGGGA24250.074.0399253
ATAGGGC16500.073.7785643
GAATAGG16300.073.2747961
CGTAGGG8600.072.688812
GGTAAGG16450.072.6066361
AGGGATG35900.071.743795
TAGGGTA8550.071.463374
ATAGAGG20050.071.061891
AGTAAGG18450.070.5979161
GAGGGAT30800.070.348774
GTAGGGC12650.069.109353
TAGAGGG50300.069.054312
GTAGGGA23500.068.20263
AGTAGGG44400.067.962042
TAAGGGA23500.067.802583
TGTAGGG22200.067.750322
TAATAGG7900.066.665241
AAGAGGG95850.066.641032
Produced by FastQC (version 0.11.5)