FastQCFastQC Report
Mon 27 Feb 2023
SRR3128205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318671
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416921.7980989972272912No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG38310.16522395803458104No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC37610.16220498725347407No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG30780.13274845806067356No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27500.11860242354348677No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT27160.1171360663069491No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25930.11183130336300406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT130950.087.3658141
GAATAGG12800.075.003631
ATAGGGC14150.074.809523
TAGGGCA11700.074.3903664
ATAGGGA20200.074.29683
CGTAGGG6350.073.350262
AGATAGG13250.072.456341
ATAGAGG15000.070.591651
ATGTAGG6800.070.5916441
AGGGATG34100.070.296595
GGTAAGG13200.070.235121
AGTAGGG37550.069.914042
TAGAGGG40350.068.793692
TAGGGTA6650.068.624764
TATAGGG20400.068.496192
GAGGGAT27500.067.576424
TAGCGGG10600.067.021052
TAAGGGA19750.066.699373
AGTAAGG13200.066.669891
ATAGCGG4100.066.5742341
Produced by FastQC (version 0.11.5)