FastQCFastQC Report
Mon 27 Feb 2023
SRR3128204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380447
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570332.3958945525777304No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG64310.2701593440223622No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT55980.23516591631739756No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC55860.23466180931564534TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT52440.22029475976570786No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT37850.15900375013600387No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG34350.14430062925156495No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26080.10955925504747636No Hit
CGTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC24630.10346796210963739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT171100.088.813661
ATAGCGG5000.080.003811
CCGTACA11400.075.028413
TCCGTAC11900.074.640422
ATCCGTA12150.073.206091
AGGGATG30950.072.429755
CGTACAC11750.071.993594
ATAGGGC13250.070.937093
GAATAGG11850.070.293741
AGAGGGC30500.069.800353
AGTACGG2900.069.780191
GTAGGGC10800.069.623443
GTACGGG4600.069.472082
AGTAGGG42050.069.180532
AGGGCAT22150.068.955495
TAGGGCA11950.068.8223344
AAGAGGG85000.068.1162952
GAGGGAT26150.067.932774
CGGGTAT2700.067.882855
AAGGGAT32400.067.882854
Produced by FastQC (version 0.11.5)