FastQCFastQC Report
Mon 27 Feb 2023
SRR3128203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665922
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690472.589985753521671No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG71990.27003790808583295No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC62160.23316511135734655TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT57050.2139972587345016No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT56390.21152156739769581No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT42270.15855677697997167No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG37270.1398015395799277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT196650.088.744631
TAGGGCA13200.076.197464
ATAGGGC15650.073.578753
AGGGATG35050.070.2644655
AGTAGGG44950.070.056062
CGTAGGG7150.069.6787642
TAGAGGG43100.069.137332
GGTAAGG15850.069.130821
GTACGGG6250.068.432442
ATAGCGG4950.068.4025651
AGAGGGC35500.068.3159263
GTAGGGC12200.068.188963
AAGAGGG89750.067.292912
GAGGGAT31400.066.758394
AGTAAGG13850.066.550511
AGGGCAT23600.066.516135
ATAGGGA23400.065.479053
GTAGGGA19750.065.443463
TCCGTAC9300.065.193972
CCGTACA9100.065.077343
Produced by FastQC (version 0.11.5)