FastQCFastQC Report
Mon 27 Feb 2023
SRR3128202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335780
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595382.548955809194359No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG57170.24475763984621837No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT52490.22472150630624457No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT50230.21504593754548804No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC47960.20532755653357768TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT37400.16011781931517524No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG31710.1357576484086686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT174700.088.224171
ATAGGGC14350.076.099933
TAGGGCA12300.072.710434
TAGGGTA5550.071.241734
AGTAGGG37000.070.605652
GTACGGG5100.070.144172
AGGGATG32700.069.4263155
GGTAAGG13050.068.9219061
TAAGGGA18900.068.737773
AGGGCAT20600.068.6790165
ATAGGGA20550.068.486913
TAGAGGG39250.067.757432
AGGGAAT23000.067.643325
GTAGGGC10950.067.4891053
AAGGGAT33150.067.162344
AGAGGGC33600.066.963293
GATAGGG33600.066.543022
TAGGGCG3050.066.361594
TATAGGG17550.065.9790652
TAGCGGG11350.065.940082
Produced by FastQC (version 0.11.5)