FastQCFastQC Report
Mon 27 Feb 2023
SRR3128201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533383
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389571.5377461678711826No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC59840.23620589543705TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG57590.22732449061196036No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG50050.19756191622032673No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCATGCAC48740.19239096496660787No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA41100.16223366147163695No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34300.13539208244469944No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT29080.11478722325049154No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27640.10910312416243417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120150.087.1906741
TAGGGCA11800.078.860584
CGTAGGG6950.077.089782
CGTACAC24200.076.9053654
CCGTACA24450.076.503443
TCCGTAC25450.075.1594242
GAATAGG14650.073.56421
GGTAAGG13800.072.9799041
ATCCGTA26650.072.579381
TAGGGTA7500.072.063174
AGTAGGG38750.071.800392
TAGGGCG3950.071.388954
ATAGGGC14950.071.361093
ATAGGGA23200.071.10433
GTAGGGT7900.070.794043
GTAGGGC10350.070.383063
AGGGCAT19750.069.485245
GTAGGGA18800.068.7466663
AGAGGGC36850.067.850173
AGGGATG32850.067.6715