FastQCFastQC Report
Mon 27 Feb 2023
SRR3128199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452747
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411091.6760391512047512No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC55940.22807081203238652TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG53990.220120542395934No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG46680.19031722391261716No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34310.139883974988044No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27530.11224149902130141No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT26970.10995834466416635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129250.087.400111
ATAGGGC12800.071.687043
CGTAGGG7100.071.578312
GAATAGG16650.071.238961
TAGGGCG3400.070.584174
TAGGGCA11200.070.584174
ATAGGGA23300.070.281233
AGATAGG14200.069.9399641
AGGGATG33350.069.900325
AGTAAGG16000.068.837831
GGTAAGG13350.068.7518841
ACGGGTA2750.068.445254
TAGAGGG43750.068.406142
AGTAGGG38800.068.279872
TATAGGG16700.067.625552
GAGGGAT28050.067.103194
CGAAGGG24050.066.915542
AGGGTAC10800.066.147465
TAGCGGG11100.066.132912
ATAGAGG16450.066.096341
Produced by FastQC (version 0.11.5)