FastQCFastQC Report
Mon 27 Feb 2023
SRR3128197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084814
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516311.6737151737511564No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC55520.17997843630118382No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG47600.15430427896138957No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43740.14179136894477268No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG36800.1192940644071247No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT31980.10366913531901761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT181700.086.142241
TAGGGCA17150.073.448654
ATAGGGC16650.073.398353
GAATAGG16250.071.487711
ATAGGGA26850.070.3734743
GGTAAGG16900.069.294761
AGTAGGG50350.068.7077262
TAGAGGG57150.068.3458252
TATAGGG24150.067.925912
CGTAGGG8650.067.923672
TAGCGGG16100.067.439342
GAGGGAT34800.067.260994
AGGGTAC13100.067.093965
ATAGCGG6450.067.083531
AGGGATG37700.066.949315
AGTAAGG19400.066.66821
GTACGGG6850.066.559252
ATAGAGG22450.065.362111
TAAGGGA25850.065.2771453
AAGAGGG110100.064.8908542
Produced by FastQC (version 0.11.5)