FastQCFastQC Report
Mon 27 Feb 2023
SRR3128196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686347
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452921.6860070571672239No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC47090.17529380977215528No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG39400.1466675749633238No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38720.1441362564106573No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG31690.11796688960882566No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT27510.10240672556449334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153700.086.251911
GAATAGG14800.070.933781
AGTAGGG45100.070.232012
ATAGCGG6300.069.49481
ATAGGGA24600.069.449343
TAGGGAT16750.067.436194
ATAACGG2100.067.253041
TAGAGGG47900.067.207412
TAGGGTA7850.067.149834
GGTAAGG15050.066.6274261
GTAGGGT9050.066.566853
AGGGATG35750.066.52685
ATAGGGC14350.066.251553
GTAGGGA20700.066.163623
AAGAGGG102400.065.9551242
ATAGAGG19400.065.7623751
GAGGGAT31650.065.429794
AGGGAAT23100.065.315355
AGTAAGG17550.065.1837161
TAAGGGA22300.065.004353
Produced by FastQC (version 0.11.5)