FastQCFastQC Report
Mon 27 Feb 2023
SRR3128195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863511
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271941.45928840774216No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGTACAG89230.4788273318483228No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC87700.47061702345733397No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG74690.40080257106075573No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG68890.369678526179883No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA55740.2991128037344561No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATG25810.1385019997198836No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT25490.13678481103680099No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT25100.134691987329294No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23330.12519378742599319No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT22520.12084715357194029No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG21900.11752010049846769No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21000.11269050732729777No Hit
GGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT19960.10710964410727922No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT19870.10662668479016224No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTGTACAGTC19070.10233371308245565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85650.086.551651
CCGTACA35750.080.977893
TCCGTAC37650.079.76232
ATCCGTA38550.078.4572451
CGTACAC37050.078.009714
TAGCGGG10450.076.003262
TAGGGCA8600.075.412344
TAGGGCG3350.074.352034
AGGGATG24100.074.101735
AGTAGGG28150.073.9583362
GTAGGGA15100.073.7620853
GTACACC39050.073.412595
TACACCC37500.072.937946
CGTAGGG4800.072.452312
AGGGCAT15100.071.894695
AGGGTAC7800.071.6991655
GAATAGG8350.070.979111
GAGGGAT25200.070.494144
TAGGGAA12400.070.115154
GTAGGGC8050.070.056293
Produced by FastQC (version 0.11.5)