FastQCFastQC Report
Mon 27 Feb 2023
SRR3128194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047641
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331701.6199128655853248No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC93950.4588206624110379No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG80450.392891136678744No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG73090.35694733598321193No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGTACAG51260.2503368510398063No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA38680.1889003003944539No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27140.13254276506477455No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT25770.1258521391200899No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT25560.12482656871980977No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT24490.11960104334695389No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23310.11383831443109411No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT23100.112812744030814No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG21580.10538956780021498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102550.085.9625241
TAGGGTA4850.081.403534
CGTAGGG4550.077.473922
AGGGCAT16400.075.946495
GAATAGG9800.074.841421
AGGGTAC9800.074.3380665
AGGGTAT10450.073.762095
TAGGGCG3050.072.427524
TAGCGGG10250.071.532992
TAGGGCA10100.071.1992954
TAAGCGG4050.070.813871
TAAGGGA17550.070.434313
ATAGGGA20250.070.327193
AGTAGGG28600.070.008252
AAGGGTA15250.069.3455054
GGTAAGG10600.069.193021
TCCGTAC25150.068.959492
AAGGGCA30850.068.101714
ATAGCGG4700.068.022811
AAGGGAC19350.068.011554
Produced by FastQC (version 0.11.5)