FastQCFastQC Report
Mon 27 Feb 2023
SRR3128193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806704
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288691.597882110185177No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC77970.4315593478511145No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG68020.3764866851459896No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG61000.33763139949875576No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGTACAG29030.1606793365155554No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24640.13638094563359576No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT23800.13173159521426864No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT22390.12392732843896953No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT21920.12132590618053649No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20940.11590166402465484No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20380.11280209707843676No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT19210.1063262161372311No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT18140.10040382929356441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88700.084.933651
TAGGGTA5800.077.863694
AGGGTAC8950.075.6279755
GTAGGGC9050.075.37223
TAGGGCA9950.075.1736764
CGTAGGG5300.074.558162
AGTAGGG27900.073.851892
AGGGCAT16050.073.50925
GCATAGG3800.073.060141
ATAGGGC11750.072.866063
ATAGCGG4450.071.905311
ATAGGGA19400.071.533923
GTAGGGT7450.071.353273
AAGGGAT27950.070.858514
GAATAGG9700.070.826081
GTAGGGA15150.070.175833
TATAGGG14600.069.919552
AGTAAGG11650.069.876681
AGGGATG24650.069.4108665
TAGCGGG10100.069.399542
Produced by FastQC (version 0.11.5)